Skip to main content
Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications

Fig. 1

GeneGuard as an example for a modular, multilayered biosafety system [32]. The plasmid system uses conditional origins of replication, auxotrophies and TA systems. Replication initiators are provided in trans by the host, minimizing the risk of replication in unintended hosts. The host lacks the essential genes thyA or dapA which are located on the plasmid, making the plasmid essential for cell survival. As a third layer the TA systems Epsilon-Zeta or Kis-Kid were used to kill the cells after losing the plasmid

Back to article page