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Table 1 Biosafety parts in the iGEM competition

From: Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications

Team Mechanism Organism Characteristics BioBrick Status a References
NTU Taida 2012 Type I TA-system E. coli SrnB-SrnC TA-system, used for plasmid maintenance BBa_K817015 Available [90]
University of Maryland 2015 Type I TA-system E. coli Hok-Sok TA-system, antibiotic free plasmid maintenance BBa_K1783001 Not available [91]
Paris Bettencourt 2012 Type II TA-system E. coli Col E2 TA-system, located on two separate plasmids BBa_K914001, Ba_K914002 Available, complicated [104]
Wageningen UR 2014 Type II TA-system E. coli Combination of the Kis-Kid and the Epsilon-Zeta TA-system to prevent horizontal gene transfer, located on two separate plasmids BBa_K1493601, BBa_K1493603 Available, complicated [105]
UC Berkeley 2008 Type II TA-system E. coli Holin-antiholin TA-system, coupled expression of lysozyme BBa_K112808 Complicated [106]
LMU Munich 2012 Sporulation induced killswitch B. subtilis mazF under control of PydfG, which is activated in germinating cells BBa_K823044 Available [113]
TU Eindhoven 2014 Timer-coupled killswitch E. coli Oscillating concentration of Spo0AP induces expression of a toxin   Conceptional [114]
UC Berkley 2007 RNase E. coli CDS of Barnase, without start codon BBa_I716211 Available [124]
Bielefeld Germany 2013 RNase E. coli CDS of Barnase BBa_K1172904 Available [77]
UC London 2012 Sequence specific self-destruction system E. coli threefold active biological containment system containing EcoRI/EcoRI methyltransferase, Holin/Anti-Holin Endolysin and Colicin-E3/Colicin Immunity E3 BBa_K729009, BBa_K729010 Not available [133]
TU Munich 2013 Sequence unspecific self-destruction system Physcomitrella patens Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC [25] BBa_K1159105 Available [138]
HKU Hongkong 2015 Sequence specific and unspecific self-destruction system E. coli CRISPR/Cas9 containment device repressed by arabinose and tryptophan BBa_K1774000 Not available [160]
Harvard 2010 Genetic fence E. coli Barnase: gene for the genetic fence, Barstar: Inhibitor of barnase −/− Conceptional [374]
Virginia 2016 Dependency on a modified amino acid E. coli N-carbobenzyloxy (CBZ)-cleavage enzyme to detach the protecting group frim amino acids BBa_K1879000 Not available [375]
Virginia 2016 Dependency on a modified amino acid E. coli Mutatnt Leucyl-tRNA synthetase −/− Conceptional [375]
Bielefeld-CeBiTec 2015 Cell free protein synthesis E. coli extract Mixture of amino acids, co factors, cell extract, NTPs, energy source, DNA template, Mg- and K-glutamate solutions, nuclease free water for cell free protein synthesis in microcentrifuge tubes or a multi-well plate −/− −/− [280]
Freiburg 2015 DiaMIX E. coli extract Cell-free expression mix for reaction in a microfluidic chamber −/− −/− [284]
Paris Saclay 2015 Microencapsulation   Silica beads    [302]
Paris Bettencourt 2012 Microencapsulation   Alginate beads    [104]
Paris Saclay 2015 RNA-Thermometer E. coli Thermosensitive cI repressor (cI857) is placed under the control of the RNA thermometer ROSE BBa_K1707013 Not available [302]
TU Delft 2008 RNA-Thermometer BradirhizobiumJaponicum, E. coli ROSE-RNA Thermometer BBa_K115001 Available [346]
TU Delft 2008 RNA-Thermometer   ForU RNA-Thermometer BBa_K115002 Available [346]
NCTU Taiwan 2011 RNA-Thermometer E. coli Promoter (LacI regulated) + RNA thermometer+vioD with RNA thermometer+tetR+double terminator BBa_K539461 Available [347]
NCTU Taiwan 2011 RNA-Thermometer E. coli Promoter (LacI regulated) + alss+ilvC+ilvD (each preceded by own RBS) and RNA thermometer+terminator BBa_K539691 Available [347]
NCTU Taiwan 2011 RNA-Thermometer E. coli a RBS (B0030) + ilvC with a RBS (B0030) + ilvD BBa_K539642 Available [347]
NCTU Taiwan 2011 RNA-Thermometer E. coli a RBS (B0030) + ilvD with RNA thermometer+tetR+terminator BBa_K539653 Available [347]
NCTU Taiwan 2011 RNA-Thermometer E. coli a RBS (B0030) + ilvC+a RBS (B0030) + ilvD with RNA thermometer+tetR+terminator BBa_K539674 Available [347]
METU Ankara 2011 RNA-Thermometer E. coli Cell destruction via lysis casette   Conceptional [376]
Cornell 2011 Light-sensor (green) E. coli CCaS and CCar are integral proteins involved in the green light-induced gene expression BBa_K597105 Not available [368]
NYMU Taipei 2014 Light sensor (blue) E. coli FixK2 blue light sensitive promoter for ccdB expression   Conceptional [377]
Minnesota 2014 Light sensor (blue)   Blue light induced promotor with kill-switch gene be Endolysin and Holin   Conceptional [378]
HNU China 2014 Light sensor (blue)   Blue light induced casp3 expression   Conceptional [379]
Braunschweig 2014 Light sensor blue   Blue light leads to the dimerization of the VVD Domains inhibiting the transcription initiation   Conceptional [380]
  1. This table summarizes biosafety systems designed by iGEM teams. Not all systems make use of BioBricks, some are based on auxotrophic strains or cell-free formulation of a system. Since only BioBricks can be submitted to the iGEM Registry of Standard Biological Parts, no entry could be cited for such systems. The status of all BioBricks was adopted from the database. Ambiguous sequencing results might lead to the classification of functional parts as ‘complicated’. Teams are listed in order of mentions in text
  2. aStatus as stated in the iGEM Registry of Standard Biological Parts. Ambiguous sequencing results might lead to the classification of functional parts as ‘complicated’