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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl2

Fig. 1

CoCl2-induced hypoxia-mimicking conditions can lead to prolonged growth inhibition in MCF-7 cells. Cell growth analysis (a) and representative fluorescence images of cell cycling marker Ki67 (red) expression (b) in MCF-7 cells treated with 100 and 300 μM CoCl2 compared to untreated control cells (day 6 of culture). Immunofluorescence analysis of Ki67 was performed after 6 days of CoCl2 treatment. Nuclei were stained with DAPI. Scale bars indicate 200 μm. c Flow cytometric analysis of MCF-7 cell cycle distribution through propidium iodide (PI) staining intensity in cells treated with 300 μM CoCl2 for 6 days compared to untreated cells (day 6 of culture). Data were analyzed by Modfit LT software (* P < 0.001 compared to untreated control)

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