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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl2

Fig. 2

Quiescent MCF-7 cells can resume growth upon removal of CoCl2. a Cell growth analysis of MCF-7 cells treated with 300 μM CoCl2 for 6 days (from day 4 to day 10) compared to untreated control cells. Cells recovered in normal growth media after the 6-day CoCl2 treatment. b Representative fluorescence images of cycling marker Ki67 (red) and nuclei (blue) in MCF-7 cells after 6 days of treatment with 300 μM CoCl2, 12 days of treatment with 300 μM CoCl2, or 6 days of treatment with 300 μM CoCl2 followed by 6 days of recovery. The untreated control consists of cells at day 4, prior to CoCl2 treatment. Scale bars indicate 200 μm

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