Fig. 5
From: A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl2
CoCl2 treatment also induces dormancy in OVCAR-3 cells. a Representative fluorescence images of HIF1α and GLUT1 expression in OVCAR-3 cells after 72 h of 100 μM CoCl2 treatment compared to untreated control cells. Scale bars indicate 200 μm. b Cell growth analysis of OVCAR-3 cells treated with 100 μM CoCl2 for 6 days (from day 6 to day 12) compared to untreated cells. Cells recovered in normal growth media after the 6-day CoCl2 treatment. c Percentages of Ki67-positive cells in untreated control, 6-day 100 μM CoCl2 treatment, and 6-day 100 μM CoCl2 treatment followed by 6-day recovery in normal growth media (* P < 0.001 compared to untreated control; # P < 0.001 compared to 6-day CoCl2 treatment). Quantification was performed with ImageJ software. d Representative fluorescence images of Ki67 (red), GLUT1 (green) and nuclei (blue) in OVCAR-3 cells in untreated control, 6-day 100 μM CoCl2 treatment, and 6-day 100 μM CoCl2 treatment followed by 6-day recovery in normal growth media. Scale bars indicate 200 μm