Fig. 7
Monocyte adhesion on HUVEC grown in full wells or only at the centre or edge of segmented wells. a The density of HUVEC, b The ratio of THP-1 monocytes to HUVEC, c the number of THP-1 and (d) representative images of Calcein-AM-stained THP-1 adhered on HUVEC (scale bar = 200 μm), all shown for the centre and edge of full or segmented wells, with and without TNF-α treatment. (UT = untreated; one-way ANOVA followed by Fisher’s Least Significant Difference test, n = 6; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)