Optimization of fermentation conditions for an Escherichia coli strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis

Table 3 The regression analysis of variance for Plackett–Burman factorial model for the yield of plasmid DNA vaccine

Code	Term	Stdized Effects	%Contribution	F-Value	p-Value
	Model			734.21	0.0287
A	Peptone(g/L)	70.30	48.18	3538.00	0.0107*
B	Yeast extract(g/L)	17.42	2.96	217.36	0.0431*
C	NaCl(g/L)	−10.49	1.07	78.81	0.0714
D	Amp(g/L)	−13.59	1.80	132.16	0.0552
E	Microelement(mL/L)	−13.82	1.86	136.70	0.0543
F	Mannitol(g/L)	47.02	21.56	1582.96	0.0160*
G	Initial pH	5.52	0.30	21.79	0.1344
H	Rotational speed(RPM)	1.02	0.010	0.74	0.5470
J	Temperature(°C)	−8.46	0.70	51.27	0.0883
K	Inoculum concentration(OD)	47.01	21.55	1582.33	0.0160*

ISSN: 1754-1611