Optimization of fermentation conditions for an Escherichia coli strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis

Table 5 Through BBD optimizing the screened culture conditions for the yield of plasmid DNA vaccine pcDNA-CCOL2A1 by the engineered Escherichia coli DH5α

Run	A-Peptone(g/L)		B-Mannitol(g/L)		C-Inoculum concentration(OD)		Plasmid yield (mg/L)
Run	Code level	Real level	Code level	Real level	Code level	Real level	Plasmid yield (mg/L)
1	1	28	−1	7	0	0.35	277.80 ± 8.38
2	1	28	0	8	− 1	0.25	293.63 ± 5.63
3	1	28	0	8	1	0.45	307.20 ± 6.81
4	0	26	−1	7	1	0.45	305.73 ± 9.32
5	0	26	1	9	−1	0.25	305.33 ± 11.90
6	0	26	0	8	0	0.35	332.53 ± 14.61
7	0	26	0	8	0	0.35	335.60 ± 10.52
8	0	26	0	8	0	0.35	339.70 ± 10.91
9	0	26	0	8	0	0.35	345.10 ± 13.60
10	0	26	−1	7	−1	0.25	285.03 ± 8.23
11	−1	24	0	8	1	0.45	309.53 ± 9.69
12	−1	24	0	8	−1	0.25	308.70 ± 10.68
13	-1	24	1	9	0	0.35	295.80 ± 10.61
14	0	26	1	9	1	0.45	302.10 ± 14.45
15	1	28	1	9	0	0.35	310.73 ± 10.71
16	0	26	0	8	0	0.35	340.97 ± 13.16
17	-1	24	-1	7	0	0.35	304.93 ± 8.88

Data are expressed as the mean ± standard deviation (SD) of 3 batches independent experiments for each strain

ISSN: 1754-1611