Fig. 1

SVF and ad-MVF isolation and scaffold seeding. a Epididymal fat pads (arrows) of a GFP⁺ C57BL/6 donor mouse. Scale bar: 10 mm. b, c Freshly isolated SVF single cells (b) and ad-MVF (c). Scale bars: 50 μm. d, e Fluorescence microscopy of bisbenzimide (blue)/propidium iodide (red)-stained SVF single cells (d) and ad-MVF-associated cells (e) for the assessment of viability (arrows = dead propidium iodide⁺ cells). Scale bars: 50 μm. f Integra® scaffold on the tip of a micro forceps directly after sample preparation. Scale bar: 2.4 mm. g, h Scheme displaying scaffold seeding with SVF single cells (blue) (g) and ad-MVF (red) (h). i Overview of the dorsal skinfold chamber window directly after implantation of an Integra® scaffold (black frames = ROIs in the border; white frames = ROIs in the center). Scale bar: 1.8 mm