Fig. 2

Intravital fluorescence microscopy of implanted scaffolds. a-f Intravital fluorescence microscopy (blue light epi-illumination with contrast enhancement by 5% FITC-labeled dextran) of SVF- (a-c) and ad-MVF-seeded (d-f) Integra® scaffolds on day 14 after implantation into full-thickness skin defects within dorsal skinfold chambers of C57BL/6 recipient mice (dotted lines = implant borders; arrows = perfused blood vessels; b, e = higher magnifications of inserts in a and d; c, f = higher magnifications of inserts in b and e). Scale bars: a, d = 2.4 mm; b, e = 500 μm; c, f = 125 μm. g-j Perfused ROIs (g, h) and FMD (i, j) in the border (g, i) and center zones (h, j) of SVF- (white circles, n = 8) and ad-MVF-seeded (black circles, n = 8) Integra® scaffolds on day 0, 3, 6, 10 and 14 after implantation, as assessed by intravital fluorescence microscopy. Means ± SEM. *p < 0.05 vs. SVF-seeded Integra® scaffolds