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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Prevascularization of collagen-glycosaminoglycan scaffolds: stromal vascular fraction versus adipose tissue-derived microvascular fragments

Fig. 5

Epithelialization of implanted scaffolds. a-f Stereomicroscopic images showing the epithelialization of implanted SVF- (a-c) and ad-MVF-seeded (d-f) Integra® scaffolds directly (a, d) as well as on day 6 (b, e) and 14 (c, f) after implantation. Scale bars: 1 mm. g Epithelialization of SVF- (white bars, n = 8) and ad-MVF-seeded (black bars, n = 8) Integra® scaffolds on day 0, 3, 6, 10 and 14 after implantation, as assessed by trans-illumination microscopy. Means ± SEM. h, i Immunohistochemical detection of the cytokeratine+ epithelial layer (arrows) covering SVF- (h) and ad-MVF-seeded (i) Integra® scaffolds. Scale bars: 300 μm. j Epithelialization of SVF- (white bars, n = 8) and ad-MVF-seeded (black bars, n = 8) Integra® scaffolds on day 14 after implantation, as assessed by immunohistochemistry. Means ± SEM

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