Skip to main content

Table 2 ECM-based matrices to induce dormancy

From: Engineered In Vitro Models of Tumor Dormancy and Reactivation

Biomaterial/ECM Characteristics Mechanism Inducing Dormancy
Collagen/Gelatin [89] Naturally occurring animal-derived biopolymer with collagen/gelatin backbone crosslinked with transglutaminase Increased stiffness resulting from increased crosslinking density of gelatin precursor
Agarose [91, 93] Plant-derived biopolymer physically crosslinked at ambient temperature Mechanical stress arising from a confining, non-adhesive matrix
Matrigel [94] Mouse-tumor derived matrix consisting of collagen, laminin, elastin and growth factors amongst other components Physical confinement in a 3D matrix
Fibrin [100, 101] Naturally occurring biopolymer in blood obtained via crosslinking of fibrinogen with thrombin Matrix stiffness
PEG [90] Synthetic bio-inert polymer that can be chemically and mechanically tuned Non-degradability and physical confinement
Silica-PEG [88] Silicate network gel formed via hydrolysis of silicon alkoxide and condensation reaction to form a porous silica network, with PEG porogen and silica nanoparticles Physical confinement in a non-degradable matrix
Collagen-PEG IPN [87] Double crosslinked network of collagen and PEG with varying PEG concentrations Physical confinement in an increasingly non-degradable matrix
PEG-protein and PEG-peptide blends [92, 105] Covalent coupling of PEG with proteins (fibrinogen) or ECM-mimetic peptides (RGDS) Controlled cell-matrix interactions
  1. Abbreviations: 3D Three-dimensional, ECM Extracellular matrix, IPN Interpenetrating network, PEG Poly(ethylene glycol);