Fig. 3

Identification of inducible promoter 39 K-regulated genes. a Immunofluorescence analysis of 39 K promoter activated foreign protein expression. p39K-DsRed plasmid co-transfection with immediate early genes and examined under a fluorescence microscope at 96 h p.i. Red represents DsRed protein expression, white represents the number of cells. The letters on the right represent cells of the corresponding early gene overexpressed or BmNPV infection. Mock represents cells not infected with BmNPV or early gene expression. b Transcription of inducible p39K-DsRed system with BmNPV immediate early genes. Transient co-expression of p39K-DsRed plasmid and immediate early gene or infected with BmNPV at 10 MOI. At 48 h p.i., total RNA was isolated from each transfected cell and quantified by RT-PCR. Each data point was determined from the mean of three independent replicates. c Relative luciferase assay of inducible p39K-DsRed system with BmNPV immediate early genes. Cells co-transfected with the Firefly luciferase and Renilla luciferase expression vector were infected or non-infected with BmNPV at 10 MOI. Cells were examined at 48 h p.i.. Each data point was determined from the mean of three independent replicates. *** represent statistically significant differences at the level of P < 0.001