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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Fig. 4

EMSA analysis of IE-1 binding to 39 K promoter region. a Electrophoretic mobility shift assay indicated that the 39 K probes bind to the recombinant IE-1 proteins. We used the competitive inhibitors unlabeled DNA probes as control and without IE-1 proteins as negative control. The shift of the positive control is indicated by a thick stripe. We detected probes 3 (− 310~ − 355) with block stripes. On the contrary, probe 1 (− 486~ − 532), probe 2 (− 386~ − 431), and probe 4 (+ 2~ + 47) were not associated with IE-1. b The EMAS detected that the probe 3 (− 310~ − 355) binds to the recombinant IE-1 proteins. The probe 3 (− 310~ − 355) probes concentrations were 1, 2, and 6 pmol/L; the IE-1 protein concentration was 0.8 μg/L; the concentrations of compete probes were 2, 20, and 100 pmol/L.

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