Fig. 2

Cultivation of U. maydis AB33P5ΔR on galacturonic acid. The strain was grown on glucose (4 g/L) and galacturonic acid (20 g/L) as carbon sources. Vertical grey dashed lines indicate depletion of glucose (11.5 h) and galacturonic acid (43 h). a Biological duplicates of oxygen transfer rate and carbon dioxide transfer rate, represented each as line and dashed line. b Concentrations of galacturonic acid and glucose, OD₆₀₀ and pH. The blue dotted line represents the assumed course of galacturonic acid concentration since the OTR indicates galacturonic acid depletion after 43 h. Culture conditions: modified Verduyn medium, 0.2 M MOPS, initial pH 6.5, 250 mL flask, filling volume 20 mL, shaking frequency 300 rpm, shaking diameter 50 mm, initial OD₆₀₀ = 0.2, T = 30 °C