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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

Fig. 7

Comparison of enzymatic activity determined from offline (DNS) and online (OTR) measurements. The hydrolytic enzymatic activity on polygalacturonic acid was determined by offline DNS assays (blue bars) or by online determination of the linear substrate consumption shown in Fig. 6 (red bars). Samples of culture supernatant were taken and analysed after cultivation times of 17 and 23 h. For offline assays, the reaction time was 4 h and the polygalacturonic acid concentration 4 g/L. Light blue bars show the galacturonic acid concentration at the beginning of the offline assay

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