Fig. 6

HIF-1α pathway activation is crucial for CD24⁺ NP cells maintenance. a-b Western blot analysis of HIF-1α expression in CD24⁺, CD24⁻ and unsorted NP cells. c Immunofluorescence staining analysis of CD24 in WT and NP-specific HIF-1α knock out mice, scale bars represent 100 μm. d-e Fluorescence activated cell sorting analysis of the percentage of CD24⁺ NP cells after Hif-1α or Vhl knock down. f Immunofluorescence staining analysis of CD24 in NP cells after Hif-1α or Vhl knock down, scale bars represent 25 μm. *P < 0.05, ***P < 0.001. P-values were analyzed by one-way ANOVA