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Table 1 Effectiveness of PEG chain on PEGylated RBCs measured by agglutination (0 indicates no agglutination). Mal = Maleimide; CnCl = Cyanuric chloride. Reproduced with permission from John Wiley and Sons [75]

From: Coatings on mammalian cells: interfacing cells with their environment

Modifying PEG Reagenta

Agglutination with

Anti-A

Anti-D

None

4+

3+

Mal-Phe-PEG-5000

4+

0

Mal-Phe-PEG-10000

2+

0

Mal-Phe-PEG-20000

1+

1+

Mal-Phe-PEG-5000 + 10,000 b

4+

0

Mal-Phe-PEG-5000 + 20,000 b

1+

1+

Mal-Phe-PEG-5000 + 10,000 + 20,000 b

3+

0

Mal-Phe-PEG-5000 and 20,000 c

0

0

CnCl-PEG-5000

4+

1+

  1. aAll the incubations were carried out in the presence of IT
  2. bThe PEG reagents of different chain lengths were present together in the reaction mixture
  3. cThe RBCs were first PEGylated with the Mal-Phe-PEG-5000. After removing the excess reagents by washing, the PEGylated RBCs were reincubated with Mal-Phe-PEG-20000 in the presence of IT
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Journal of Biological Engineering

ISSN: 1754-1611