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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in Saccharopolyspora erythraea

Fig. 2

SACE_5754 negatively regulates erythromycin production in S. erythraea A226. a Genetic organization of ery cluster, SACE_5754 and its adjacent gene SACE_5753 in S. erythraea A226. b Purification of His6-tagged SACE_5754. c EMSAs of the interaction of probes PeryBVI, PeryAI-BIV, PeryBI-BIII, PeryCI-ermE, PeryK and P5753–5754 with purified His6-SACE_5754 protein. d Effect of SACE_5754 deletion on transcription levels of ery cluster, SACE_5753 and SACE_5754. qRT-PCR was used to quantify the amounts of transcripts in A226 and ΔSACE_5754 cultured for 48 h in liquid R5 medium. Mean values of three replicates are shown, with the standard deviation indicated by error bars.*p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant

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Journal of Biological Engineering

ISSN: 1754-1611