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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in Saccharopolyspora erythraea

Fig. 3

SACE_5754 directly represses the expression of SACE_0388, SACE_3599 and SACE_6149. a Genetic organization of candidate target genes of SACE_5754. b EMSAs with SACE_5754 and P0388–0389. c EMSAs with SACE_5754 and P3599. d EMSAs with SACE_5754 and P6148–6149. e Effect of SACE_5754 deletion on transcription levels of related genes. qRT-PCR was used to quantify the amounts of transcripts in A226 and ΔSACE_5754 cultured for 48 h in liquid R5 medium. Mean values of three replicates are shown, with the standard deviation indicated by error bars. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant

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