Fig. 4
Analysis of the SACE_5754-binding sites. a DNase I footprinting assay of SACE_5754-binding site in the P0388–0389. Upper fluorogram: control reaction without protein. Protection regions were acquired with increasing concentrations (0.5 μM and 0.2 μM) of His6-SACE_5754 protein. b SACE_5754 binds to the probe P1and mutated probe P2 and P3 by EMSAs. c DNase I footprinting assay of SACE_5754-binding site in the P3599. Upper fluorogram: control reaction without protein. Protection regions were acquired with increasing concentrations (0.2 μM and 0.5 μM) of His6-SACE_5754 protein. d SACE_5754 binds to the probe P4 and mutated probe P5 and P6 by EMSAs. e DNase I footprinting assay of SACE_5754-binding site in the P6148–6149. Upper fluorogram: control reaction without protein. Protection regions were acquired with increasing concentrations (0.05 μM and 0.2 μM) of His6-SACE_5754 protein. f SACE_5754 binds to the probe P5 and mutated probe P6 and P7 by EMSAs