Fig. 3

In a bioreactor model, addition of TNFα and IL-β1 or PGE2 enabled proliferation of MDA-MB-231BRMS1 cells that were otherwise growth arrested as indicated via Ki67 staining. Fluorescence images of cells stained for Ki67 in (a) untreated control, (b) TNFα and IL-β1 treatment, (c) PGE2 treatment, and (d) TNFα, IL-1β and AH6809 (PGE2 receptor inhibitor) treatment conditions. White arrows indicate positive nuclear Ki67 staining. Scale bar = 20 μm. Figure taken from [63] and reprinted with permission of Springer Nature