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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Modelling cardiac fibrosis using three-dimensional cardiac microtissues derived from human embryonic stem cells

Fig. 1

Characterization of CMs differentiated from hESCs. a Representative morphology of undifferentiated hESCs and CMs differentiated from hESCs after 10 days. Scale bars, 100 μm. b Flow cytometry plots of cardiac-specific marker cardiac troponin T (cTnT)+ cells. c Quantitative RT-PCR of hESC-specific marker (POU5F1) and CM-specific markers (TNNT2, TBX5, MYH6, MYH7, and ANF) in undifferentiated hESCs and differentiated CMs. Data are the means±SD of three independent experimental replicates (n = 3). **p < 0.01 d Representative immunofluorescent staining of hESC-derived CMs for cardiac-specific transcription factors (GATA4, MESP1, and NKX2.5). Scale bars, 10 μm. e Immunofluorescent staining for sarcomeric proteins (sarcomeric-alpha actinin (SAA), cTnT, MYL2, and MLC2A) and junctional protein (Cx43). Nuclei were stained with DAPI (blue). White arrow indicated Cx43-positive gap junction. Scale bars, 10 μm

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