Fig. 2From: Modelling cardiac fibrosis using three-dimensional cardiac microtissues derived from human embryonic stem cellsCharacterization of MSCs derived from hESCs. a Representative morphology of differentiated MSCs and immunofluorescence staining for MSC-specific markers (CD105, STRO1, and CD44). Nuclei were stained with DAPI (blue). Scale bars, 100 μm. b Histograms of flow cytometry analysis for MSC surface markers (CD73 and CD44). The percentage of CD73+ and CD44+ cells in the total cell population. (c) qRT-PCR analysis of MSC markers (Endoglin (ENG; CD105), Ecto-5-prime-nucleotidase (NT5E; CD73), and CD44) in undifferentiated hESCs and MSCs differentiated from hESCs. Data are the means±SD of three independent experimental replicates (n = 3). **p < 0.01Back to article page