Fig. 3From: Modelling cardiac fibrosis using three-dimensional cardiac microtissues derived from human embryonic stem cellsImportance of MSCs in generating 3D cardiac microtissue. (a) Representative image of CMs and MSCs differentiated from the same hESC line when co-cultured as 3D spheroids for day 1 and 7. Cardiac spheroid formation was dependent on the ratio of MSCs to CMs. Scale bars, 100 μm. (b) Average diameters of cardiac spheroids. Data are the means±SD of replicates (n ≥ 5). **p < 0.01. (c) Representative raw traces of the microelectrode array (MEA) recording from control CMs and CMs co-cultured with 5, 10 and 20% MSCs on day 6 after seeding. (d) The representative traces of averaged field potential (FP) recorded by MEA in the control CMs (n = 14) and CMs co-cultured with 5% (n = 37), 10% (n = 49) and 20% MSCs (n = 48). Open triangle indicates the peak of FP and bilateral arrow indicates the field potential duration (FPD). (e) FPD obtained from the control CMs (n = 14) and CMs co-cultured with 5% (n = 37), 10% (n = 49) and 20% MSCs (n = 48). **p < 0.01, *p < 0.05Back to article page