Fig. 5

Immunohistochemical assessment of vascularization by vessel formation, functional vascular endothelial cell count, and vascular smooth muscle cell count 28 days after myocardial infarction reperfusion injury. a images of CD31 and Alexa Fluor 488 (green), SMA and Alexa Fluor 594 (red), and DAPI (blue). Vascular smooth muscle cells were characteristic of SMA+ associated with CD31+. Scale bar represents 100 μm. Quantification of immunohistochemical assessment of vascularization by b vascular smooth muscle cell counts and ratio of vascular smooth muscle cells to functional vascular endothelial cells, c vessel counts with vascular smooth muscle cells, and d ratio of vessels with vascular smooth muscle cells to vessels with functional vascular endothelial cells. n = 7–12, error bars represent standard error of the mean and * indicates p < 0.05