Direct cell reprogramming for tissue engineering and regenerative medicine

Grath, Alexander; Dai, Guohao

doi:10.1186/s13036-019-0144-9

Table 1 Summary of reprogramming factors and transdifferentiated cell types

From: Direct cell reprogramming for tissue engineering and regenerative medicine

Cell Source	Transdifferentiation Method	Target Cell Type	Reprogramming Factors	References
Human Adult Dermal Fibroblast	Viral Vectors	Neurons	Brn2, Mty1l, miRNA-124	Ambasudhan et al. (2011) [22]
Human Adult Peripheral Blood Mononuclear Cells	Electroporation		Ascl1, Brn2, Myt1l, Ngn2	Tanabe et al. (2018) [27]
Human Striatum Astrocytes	Viral Vectors		Ascl1, Brn2, Myt1l	Torper et al. (2013) [40]
Murine Embryonic and Postnatal Fibroblasts	Viral Vectors		Ascl1, Brn2, Myt1l	Vierbuchen et al. (2010) [17]
Murine Bone Marrow Stromal Cells	Pharmacological Agents		Dimethylsulphoxide, butylated hydroxy-anisole, KCl, valproic acid, forskolin, hydrocortisone, insulin	Zurita et al. (2008) [41]
Human Neonatal Fibroblasts	Viral Vectors	Hepatocytes	Foxa2, Hnf4α, C/EBPβ, c-Myc	Kogiso et al. (2013) [23]
Human Embryonic Fibroblasts	Viral Vectors		Hnf1α, Hnf4α, Foxa3	Huang et al. (2014) [24]
Murine Pancreatic Cells	Pharmacological Agents		Dexamethasone, oncostatin M	Shen et al. (2003) [33]
Human Adult Fibroblasts	Viral Vectors	Endothelial Cells	ETV2	Morita et al. (2014) [4]
Murine Amniotic Cells	Viral Vectors		Sox17	Schachterle et al. (2017) [26]
Human Newborn Dermal and Lung Fibroblasts	Viral Vectors Pharmacological Agents		Oct4, Sox2, KLF4, c-Myc bFGF, βME	Margariti et al. (2012) [25]
Human Newborn Foreskin Fibroblasts	Pharmacological Agents		Polyinosinic:polycytidylic acid	Sayed et al. (2015) [12]
Murine Embryonic Fibroblasts	Pharmacological Agents	Skeletal Myocytes	5-azacytidine	Kaur et al. (2014) [13]
Murine Embryonic Fibroblasts	CRISPR/dCas9		Myod1	Chakraborty et al. (2014) [8]
Human Dermal Fibroblasts	Viral Vectors Pharmacological Agents		Myod1 SB431542, Chir99021, EGF, IGF1	Boularaoui et al. (2018) [58]
Human Dermal Fibroblasts	Pharmacological Agents	Chondrocytes	Cartilage-derived morphogenetic protein 1	Yin et al. (2010) [61]
Mouse Dermal Fibroblast	Viral Vectors		c-Myc, KLF4, Sox9	Outani et al. (2013) [62]
Murine Adult Pancreatic Exocrine Cells	Viral Vectors (in situ)	Pancreatic β-Cells	Pdx1, Ngn3, Mafa	Zhou et al. (2008) [64]
Human Pancreatic Exocrine Cells	Viral Vectors		MAPK, STAT3	Lemper et al. (2015) [65]
Murine Cardiac Fibroblasts	Viral Vectors (in situ)	Cardiomyocytes	Gata4, Mef2c, Tbx5	Qian et al. (2012) [75]
Murine Bone Marrow Mesenchymal Stem Cells	Pharmacological Agents		5-azacytidine, Zebularine	Naeem et al. (2013) [14]
Murine Cardiac Fibroblasts	Pharmacological Agents		miRNA-1, miRNA-133, miRNA-208, miRNA-499	Jayawardena et al. (2015) [85]
Murine Myoblasts	CRISPR/Cas9	Adipocytes	Myod1	Wang et al. (2017) [28]
Human Skeletal Muscle Fibroblasts	Pharmacological Agents		Dexamethasone, 1-methyl-3-isobutylxanthine, PPARγ agonists	Agley et al. (2013) [34]
Human Subcutaneous Adipocytes	Pharmacological Agents	Osteoblasts	Calcitriol, dexamethasone, ascorbic acid, and beta-glycerophosphate	Justesen et al. (2004) [35]
Murine Adipose Tissue-Derived Stem Cells	Viral Vectors		Runx2	Zhang et al. (2006) [86]
Murine Preadipocytes	Viral Vectors		Runx2, MKP-1	Takahashi et al. (2011) [87]

Back to article page