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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Polymer-based controlled-release fed-batch microtiter plate – diminishing the gap between early process development and production conditions

Fig. 1

Principle of fed-batch microtiter plate (FeedPlate®). a Cross section view of a 96-square-well fed-batch microtiter plate with the release system at the bottom of each well. b Enlarged cross section view of one well (not in scale). Embedded glucose crystals inside of the silicone matrix serve as nutrient reservoir. The mechanism of the glucose release is illustrated: 1) Water diffuses into the silicone matrix and creates a small pore with highly concentrated glucose solution. 2) Due to osmotic pressure, more water is forced into the cavity. 3) At some point, cracks and channels are established in the silicone matrix, which is followed by the release of the glucose solution into the culture medium. For release and cultivation experiments, the microtiter plate was placed in a temperature and humidity controlled incubation hood and shaken at 970 rpm with 3 mm shaking diameter

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