Fig. 2
Amount of glucose released per well over time of a 96-square-well polymer-based fed-batch microtiter plate. Glucose release into a selection of commonly applied complex (LB, YP, TB) and synthetic media (PBS, Syn6-MES, Wilms-MOPS) is depicted. All data points are mean values of measurements of three individual wells. Error-bars indicate the respective standard deviation. a 25 °C b 37 °C c 45 °C d Temperature dependency of the amount of released glucose in all six investigated media after 48 h. Experimental conditions: Humidity = 80%, shaking frequency = 970 rpm, shaking diameter = 3 mm, VL,96 = 1000 μL/well. To avoid unintended growth of contaminants, 0.2 g/L NaN3 was added to the medium