Skip to main content
Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Polymer-based controlled-release fed-batch microtiter plate – diminishing the gap between early process development and production conditions

Fig. 3

Amount of glucose released per well of a 96-square-well fed-batch microtiter plate into Wilms-MOPS medium. Lines indicate linear fits for each condition. Data points in brackets are not considered for fitting. All data points are mean values of measurements of three individual wells. Error-bars indicate the respective standard deviation. Except for the investigated parameter, all other parameters were kept constant as indicated at the bottom of each subfigure. a Influence of varying osmotic concentration on the total amount of glucose released by changing the MOPS buffer concentration (0, 50, 100, 200 mM). Additionally, 200 mM MOPS is replaced by NaCl to rule out any influence of MOPS itself on the glucose release. b Influence of varying pH-values on the total amount of glucose released. For the pH range from 6 to 8, 200 mM MOPS buffer was applied, for pH 4.5 and 5, 200 mM MES buffer was applied. c Influence of varying temperature (25, 37, 45 °C) on the total amount of glucose released. d Influence of varying nitrogen-sources (Urea, KNO3, NH4NO3, NH4Cl, (NH4)2SO4) on the total amount of glucose released. The molarity of nitrogen was constant (105 mMN) for all nitrogen sources. General experimental conditions: Humidity = 80%, shaking frequency = 970 rpm, shaking diameter = 3 mm, VL,96 = 1000 μL/well. No initial glucose was provided in the medium. No biology was applied. To avoid unintended growth of contaminants, 0.2 g/L NaN3 was added to the medium

Back to article page