Fig. 4
Glucose release rate per well of a 96-square-well fed-batch microtiter plate into Wilms-MOPS medium. For a, b and c each data point corresponds to the slope of each linear fit from Fig. 3a, b and c, respectively. For d the data points correspond to the glucose release derived from Additional file 1. Except for the investigated parameter, all other parameters were kept constant. The solid lines represent the respective equation. a Glucose release in dependency of osmotic concentration of the medium. b Glucose release in dependency of pH of the medium. c Glucose release in dependency of temperature. d Glucose release in dependency of ammonia concentration in the medium. General experimental conditions: Humidity = 80%, shaking frequency = 970 rpm, shaking diameter = 3 mm, VL,96 = 1000 μL/well