Fig. 6
Batch and fed-batch cultivation of Hansenula polymorpha RB11 pC10-FMD GFP in 96-square-well microtiter plates. Data correspond to conventional batch microtiter plate (black, open symbols) and in polymer-based fed-batch microtiter plate (red, solid symbols) in Syn6-MES medium. All offline measured data points are mean values of measurements of four individual wells. Exception: Oxygen transfer rate and pH are measured in duplicates. A) Oxygen transfer rate (OTR) and respiratory quotient (RQ) B) Cell dry weight (CDW, squares) and pH (stars); C) Green fluorescence protein (GFP, triangle) and GFP-yield per glucose (cross); D) Measured glucose (circle) and acetate (diamond) concentration. Dotted lines represent the calculated total glucose concentration available for the microorganisms until the respective time. For the calculation (Equation 1), the initial medium properties were applied. Cultivation conditions: initial biomass concentration: 0.35 g/L, temperature = 37 °C; pH0 = 6; shaking frequency: n = 970 rpm; shaking diameter: d = 3 mm; culture volume VL,96 = 600 μL/well; initial glucose concentration cS_Batch = 10 g/L, cS_FedBatch = 0 g/L. Glucose was used as sole carbon source