Fig. 7
Batch and fed-batch cultivation of Escherichia coli BL21 (DE3) pRhotHi-2-EcFbFP in 96-square-well microtiter plates. Data correspond to conventional batch microtiter plate (black, open symbols) and in commercially available polymer-based fed-batch microtiter plate (red, solid symbols) in Wilms-MOPS medium. All offline measured data points are mean values of measurements of four individual wells, the error bars indicate the standard deviation. Exception: Oxygen transfer rate (OTR) and pH are measured in duplicates. a Oxygen transfer rate (OTR) b Cell dry weight (CDW, squares) and pH (stars); c Flavin mononucleotide binding fluorescent protein (FbFP, triangle) and FbFP-Yield per glucose (cross); d Measured glucose (circle) and acetate (diamond) concentration. Dotted lines represent the calculated total glucose concentration (Eq. 1) available for the microorganisms until the respective point of cultivation. For the calculation, the initial medium properties were applied. Cultivation conditions: initial biomass concentration: 0.11 g/L, temperature = 37 °C; pH0 = 7.5; shaking frequency: n = 970 rpm; shaking diameter: d = 3 mm; culture volume VL,96 = 600 μL/well, initial glucose concentration cS_Batch = 20 g/L, cS_FedBatch = 0 g/L. Glucose was used as sole carbon source