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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits

Fig. 3

a) Postulated truncated cI with non-natural start codons (T1-T8). T9 is the AUG start codon after 40th codon. b) Frame-shifted cI in Fs2 NOT gate construct is replaced by individual truncated cIs (T1-T9) without changing the PLtetO-1 promoter, native RBS and the linker region between RBS and ORF, and co-transformed with PR-EGFP carrying plasmid (p15A Ori) to create 9 NOT gates. The start codons of frame-shifted cI and truncated cIs are in red and the stop codons (UGA) are also shown. c) The fold change in EGFP expression of the NOT gates with various truncated cIs, T1-T9 between output ‘ON’ (input OFF; 0 ng/ml aTc) and ‘OFF’ states (input ON; 200 ng/ml aTc)

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