Table 1 Various NOT gate constructs from wild type (W), frame-shifted (Fs) and truncated versions (T) of cI gene (cI)

Fs1

frame-shifted cI

pUC

pUC

Fs2

frame-shifted cI

pUC

p15A

Fs3

frame-shifted cI

p15A

pUC

Fs4

frame-shifted cI

ColE1

ColE1

Fs5

frame-shifted cI

ColE1

p15A

Fs6

frame-shifted cI

p15A

ColE1

Fs7

frame-shifted cI

p15A

p15A

W1

wild type cI

pUC

pUC

W2

wild type cI

pUC

p15A

W3

wild type cI

p15A

pUC

W4

wild type cI

ColE1

ColE1

W5

wild type cI

ColE1

p15A

W6

wild type cI

p15A

ColE1

W7

wild type cI

p15A

p15A

T1

TruncatedcI(T1^a/cI-G, starting from glycine)

pUC

p15A

T2

TruncatedcI(T2^a/ cI-T3, starting from threonine 3)

pUC

p15A

T3

TruncatedcI(T3^a/ cI-K4, starting from lysine 4)

pUC

p15A

T4

TruncatedcI(T4^a/ cI-K5, starting from lysine 5)

pUC

p15A

T5

Truncated cI(T5^a/ cI-K6, starting from lysine 6)

pUC

p15A

T6

TruncatedcI(T6^a/ cI-L8, starting from leucine 8)

pUC

p15A

T7

TruncatedcI(T7^a/ cI-T9, starting from threonine 9)

pUC

p15A

T8

TruncatedcI(T8^a/ cI-E11, starting from glutamate 11)

pUC

p15A

T9

TruncatedcI(T9^a/ cI-M41, starting from methionine 41)

pUC

p15A