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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Rational protein design of Bacillus sp. MN chitosanase for altered substrate binding and production of specific chitosan oligomers

Fig. 5

Production of D4 with CSN-W118A at a semitechnical scale. One g of chitosan polymer DA 1.5% was hydrolyzed with 0.367 μM of CSN-W118A for 51.5 h at 37 °C. a A part of the elution profile for the separation of the chitosan oligomers by SEC. The products were identified using UHPLC-ESI-MS1 measurements. Fractions containing D4 were combined and washed, and the amount and purity of the product were determined by (b) UHPLC-ELSD-ESI-MS1 measurements

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Journal of Biological Engineering

ISSN: 1754-1611