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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Rational protein design of Bacillus sp. MN chitosanase for altered substrate binding and production of specific chitosan oligomers

Fig. 7

Differences in the pattern of acetylation between CSN, CSN-Y270A, CSN-E59A, and CSN-W118A for A1D3. The results are obtained from the reactions described in Fig. 6. The most prevalent monoacetylated tetramer product is indicated in bold for each enzyme. A combination of quantitative UHPLC-ELSD-ESI-MS1 and UHPLC-ELSD-ESI-MS2 measurements was used to determine the absolute amount of A1D3 in the hydrolysates and the relative frequency of each possible pattern. The experiments were performed as triplicates with one batch of each enzyme, the standard deviation is indicated

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Journal of Biological Engineering

ISSN: 1754-1611