Fig. 2

Microcarrier Screening. a Attachment over 24 h of eCB-MSCs on Cytodex 3, Cytodex 1, Cultispher S, Synthemax II and Enhanced Attachment microcarriers in 6-well plates. Statistical significance compares the attached cells density at each time point (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) b The eCB-MSCs on microcarriers at 24 h. Cultispher S was stained with Calcein-AM/ ethidium homodimer-1, the other microcarriers were stained with crystal violet. Scale bars are 200 μm. c Attached cell density over 7 days for Cytodex 3, Cultispher S, and static T-flasks. Error bars are standard error of the mean. Statistical significance compares the attached cells density at each time point