Fig. 3

Inoculation Phase. a Attachment over 24 h of eCB-MSCs to static T-flasks and Cytodex 3 microcarriers in bioreactors. (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) b Attached cells, alive cells in suspension, dead cells, and total cells of eCB-MSCs over 24 h in bioreactor culture. Statistical significance compares the attached cells to the alive cells in suspension. c Attached cells, alive cells in suspension, dead cells, and total cells of eCB-MSCs over 24 h in static culture. Statistical significance compares the attached cells to the alive cells in suspension. d Attached cell densities on microcarriers in bioreactors passaged from T-flasks at 3 different densities over a 7 day period. Statistical significance compares the medium inoculation condition to the high and low inoculation conditions. e Attached cell densities and f Fold increase of eCB-MSCs on microcarriers in bioreactors with inoculation densities of 2 cells/MC, 4 cell/MC and 8 cells/MC. Statistical significance compares the 2 cells/MC condition to the 4 cells/MC and 8 cells/MC conditions