Fig. 4

Expansion Phase. a Attached cell density over a 7 day period of eCB-MSCs grown in static and bioreactors in new and original medium. Statistical significance compares the new medium to the original medium within the bioreactor and static conditions. (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) b Attached cell density in static and bioreactor with no medium change over a 6 day period. The medium from the culture was used for media analysis. c The percentage of bFGF remaining in the culture medium on each day of expansion in bioreactor and static culture. d The concentration (g/L) of glucose and lactate in the culture medium on each day of expansion in bioreactor and static culture. e Attached cell density in bioreactor and static culture, with and without medium change. Statistical significance compares the medium change to the no medium change condition within bioreactor and static culture. f Attached cell densities of eCB-MSCs grown in bioreactors run at 40 rpm, 60 rpm, and 80 rpm. Statistical significance compares to the 80 rpm condition