Fig. 5

GABA production by whole-cell biocatalysis with enigneered E. coli strains. (a) Bioconversion of 2 M MSG/L-Glu to GABA in the sodium acetate buffer (pH 4.6) and water; (b) Effect of L-Glu concentration on GABA production. Conversions were performed at 37 °C for 1 h with a cell density of OD₆₀₀ 20 and PLP concentratin of 0.2 mM in water. Data are shown as the mean ± standard deviation (n = 3). The different letters indicate significant differences among samples at a significant level of 0.05 (p < 0.05), and the same letters indicate no significant differences