Journal of Biological Engineering

Table 2 Kinetic parameters for StGAD, SsGAD, ScGAD and GadB

From: Identification of new glutamate decarboxylases from Streptomyces for efficient production of γ-aminobutyric acid in engineered Escherichia coli

Kinetic parameters	Streptomyces GADs			GadB
Kinetic parameters	StGAD	SsGAD	ScGAD	GadB
V_max (μmol·min^− 1·mg^− 1)	0.226 ± 0.01^d	23.42 ± 1.57^b	29.94 ± 2.16^a	12.53 ± 0.72^c
K_m (mM)	7.67 ± 0.55^c	35.17 ± 2.12^a	23.25 ± 2.07^b	35.83 ± 2.40^a
k_cat (s^− 1)	0.20 ± 0.02^d	21.96 ± 2.08^b	28.08 ± 1.99^a	11.00 ± 0.88^c
k_cat/K_m (mM^− 1·s^− 1)	0.026 ± 0.003^d	0.62 ± 0.04^b	1.21 ± 0.09^a	0.31 ± 0.02^c

Data are shown as the mean ± standard deviation (n = 3). The different letters indicate significant differences among samples at a significant level of 0.05 (p < 0.05), and the same letters indicate no significant differences

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ISSN: 1754-1611

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