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Table 2 Kinetic parameters for StGAD, SsGAD, ScGAD and GadB

From: Identification of new glutamate decarboxylases from Streptomyces for efficient production of γ-aminobutyric acid in engineered Escherichia coli

Kinetic parameters Streptomyces GADs GadB
StGAD SsGAD ScGAD
Vmax (μmol·min− 1·mg− 1) 0.226 ± 0.01d 23.42 ± 1.57b 29.94 ± 2.16a 12.53 ± 0.72c
Km (mM) 7.67 ± 0.55c 35.17 ± 2.12a 23.25 ± 2.07b 35.83 ± 2.40a
kcat (s− 1) 0.20 ± 0.02d 21.96 ± 2.08b 28.08 ± 1.99a 11.00 ± 0.88c
kcat/Km (mM− 1·s− 1) 0.026 ± 0.003d 0.62 ± 0.04b 1.21 ± 0.09a 0.31 ± 0.02c
  1. Data are shown as the mean ± standard deviation (n = 3). The different letters indicate significant differences among samples at a significant level of 0.05 (p < 0.05), and the same letters indicate no significant differences
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Journal of Biological Engineering

ISSN: 1754-1611