Table 3 Primers for amplifying the GAD genes from E. coli and Streptomyces
Gene | Primers | Restriction site |
|---|---|---|
gadB | Forward: 5′-CGCCATATGGATAAGAAGCAAGTAACG-3′ | NdeI |
Reverse: 5′-CCCTCGAGTCAGGTATGTTTAAAGCTGTT-3′ | XhoI | |
Stgad | Forward: 5′-AACATATGGCTCTCCACAAGACGAAGGA-3′ | NdeI |
Reverse: 5′-AAAAGCTTTTAGTGGTGGAAGCCGGAGCGGGGA-3′ | HindIII | |
Ssgad | Forward: 5′-AACATATGGCCTTGTACAAGGGCACCG-3′ | NdeI |
Reverse: 5′-AAAAGCTTTTAGTGGTGGAAGCCGGCGCGGACC-3′ | HindIII | |
Scgad | Forward: 5′-AACATATGCCACTCCACCAAGGCGCGGACA-3′ | NdeI |
Reverse: 5′-AAAGCTTTTAGTGGTGGAAGGCGGTGGCGGCC-3′ | HindIII |