Skip to main content

Advertisement

Table 3 Strains and plasmids in this study

From: Systems metabolic engineering of Corynebacterium glutamicum for the bioproduction of biliverdin via protoporphyrin independent pathway

Strains and plasmids Relevant characteristics References
Strains
E. coli DH5α F−, deoR, endA1, gyrA96, hsdR17(rk − mk+), recA1, relA1, supE44, thi-1, Δ (lacZYA-argF) U169, (Phi80lacZdelM15) Invitrogen
C. glutamicum ATCC 13032 Wild type ATCC
C. glutamicum ATCC 13826 Wild type; industrial glutamate producers ATCC
 HM001 C. glutamicum ATCC 13826 strain with plasmids pEKEx2 + pMTZ This work
 HM002 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ This work
 HM003 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hemN This work
 HM004 C. glutamicum ATCC 13826 strain with plasmids pMTZ This work
 HM005 C. glutamicum ATCC 13826 strain with plasmids pMTZ-hemH This work
 HM006 C. glutamicum ATCC 13826 strain with plasmids pMTZ-hemQNAT This work
 HM007 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hemQNAT This work
 HM008 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hemQ This work
 HM009 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemALQ + pMTZ This work
 BV001 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hmuO This work
 BV002 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemALQ + pMTZ-hmuO This work
 BV003 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemALO + pMTZ-hemQ This work
 BV004 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hemQO This work
 BV005 C. glutamicum ATCC 13826 strain with plasmids pEKEx2-hemAL + pMTZ-hemOQ This work
Plasmids
 pPsADHα ZeoR; PADH2, Pichia pastoris integration vector [50]
 pEKEx2 KanR; Ptac, lacIq, pBL1 oriVC. glutamicum; C. glutamicum / E. coli shuttle vector [51]
 pMT-tac KanR; Ptac, lacIq, pCG1 oriVC. glutamicum; C. glutamicum / E. coli shuttle vector [52]
 pMTZ ZeoR; Ptac, lacIq, pCG1 oriVC. glutamicum; C. glutamicum / E. coli shuttle vector This work
 pEKEx2-hemAL pEKEx2 carrying hemA from S. typhimurium and hemL from E. coli [41]
 pEKEx2-hemALQ pEKEx2 carrying hemA from S. typhimurium, hemL from E. coli and hemQ from C. glutamicum with an exchange of GTG start codon of hemQ to ATG This work
 pEKEx2-hemALO pEKEx2 carrying hemA from S. typhimurium, hemL from E. coli and hmuO from C. glutamicum This work
 pMTZ-hemN pMTZ carrying hemN from C. glutamicum This work
 pMTZ-hemH pMTZ carrying hemH from C. glutamicum This work
 pMTZ-hemQNAT pMTZ carrying hemQ from C. glutamicum This work
 pMTZ-hemQ pMTZ carrying hemQ from C. glutamicum with an exchange of GTG start codon of hemQ to ATG This work
 pMTZ-hmuO pMTZ carrying hmuO from C. glutamicum This work
 pMTZ-hemQO pMTZ carrying hemQ from C. glutamicum with an exchange of GTG start codon of hemQ to ATG and hmuO from C. glutamicum This work
 pMTZ-hemOQ pMTZ carrying hmuO from C. glutamicum and hemQ from C. glutamicum with an exchange of GTG start codon of hemQ to ATG This work