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Table 4 Primers used in this study

From: Systems metabolic engineering of Corynebacterium glutamicum for the bioproduction of biliverdin via protoporphyrin independent pathway

Primers Sequences (5′ → 3′)a
Zeo-F CCCGTCGACGTTGACAATTAATCATCGGCATAG
Zeo-R ATTACGTAGTGTCAGTCCTGCTCCTC
ALQ-F GCCGGATCCAAGGAGATATAGATGAGCGAGCTCGATATTAAACAG
ALQ-R CGCGGTACCTTAAGGAAGAACCTTAATCAGATCTGC
ALO-F CCCGGATCCAAGGAGATATAGATGACAAGCATTATTGCAAGCAACAG
ALO-R CCCGGTACCTTAAGCAAGAGCCTGAAAAACTTGCTG
N-F GGGATCGATATGTCAGTTTTTGGTGTGTATATTC
N-R CCCGCGGCCGCTTAGTCTTCTTCACTAAGCAAAATG
H-F GGGATCGATATGAATGAACGCACATCGGATG
H-R AAAGCGGCCGCCTAGTTGGCAGCTGGCG
QNAT-F CCCATCGATGTGAGCGAGCTCGATATTAAAC
QNAT-R GGGGCGGCCGCTTAAGGAAGAACCTTAATCAGATCTGC
Q-F CCCATCGATATGAGCGAGCTCGATATTAAACAG
Q-R CCAGCGGCCGCTTAAGGAAGAACCTTAATCAGATCTGCAATG
O-F GCGATCGATATGACAAGCATTATTGCAAGC
O-R GGGGGATCCTTAAGCAAGAGCCTGAAAAACTTGCTGATT
QO-F CCCGCGGCCGCAAGGAGATATAGATGACAAGCATTATTGCAAGCAA
QO-R GAGGCGGCCGCTTAAGCAAGAGCCTGAAAAACTTG
OQ-F CCCGGATCCAAGGAGATATAGATGAGCGAGCTCGATATTAAACAGC
OQ-R GGGGCGGCCGCTTAAGGAAGAACCTTAATCAGATCTGCAATGT
  1. aThe underlined letters mean the restriction site and the letters in bold represent the ribosome binding site (RBS) added artificially