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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Analysis of the stability and affinity of BlaR-CTD protein to β-lactam antibiotics based on docking and mutagenesis studies

Fig. 2

Purification of single point mutants and disulfide bond mutants (a) and multipoint mutants of BlaR-CTD protein (b). a Lanes 1–11 were the purified proteins of BlaR-CTD, A138E, Q147K, I188K, S190Y, V197D, S19C/G24C, R50C/Q147C, S76C/L96C, S135C/S145C, and E183C/I188C, respectively. b Lanes 1–13 were the purified proteins of A138E/S19C/G24C, Q147K/S19C/G24C, I188K/S19C/G24C, S190Y/S19C/G24C, V197D/S19C/G24C, A138E/R50C/Q147C, I188K/R50C/Q147C, S190Y/R50C/Q147C, V197D/R50C/Q147C, A138E/E183C/I188C, Q147K/E183C/I188C, S190Y/E183C/I188C, and V197D/E183C/I188C, respectively. M, protein mass marker

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