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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Analysis of the stability and affinity of BlaR-CTD protein to β-lactam antibiotics based on docking and mutagenesis studies

Fig. 3

Stability of BlaR-CTD and mutant proteins. The wild-type BlaR-CTD protein and its mutants, I188K I188K/S19C/G24C, A138E/R50C/Q147C and S190Y/E183C/I188C were respectively stored at − 20 °C and − 4 °C for 6 months and at 25 °C and 37 °C for 30 days. At indicated times, each protein was taken to react with HRP-AMP. Residual activity (%) = OD value (after storage)/ OD value (before storage) × 100%

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Journal of Biological Engineering

ISSN: 1754-1611