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Table 1 Strains and plasmids used in this study

From: Systematic analysis of the effects of different nitrogen source and ICDH knockout on glycolate synthesis in Escherichia coli

Name Relevant genotype Reference
Strains
 MG1655(DE3) fhuA2 [51]
 Mgly5 MG1655(DE3)ΔldhAΔglcBΔaceBΔaldAΔglcDEF [5]
 Mgly6 MG1655(DE3)ΔldhAΔglcBΔaceBΔaldAΔglcDEFΔicd This study
 Mgly534 Mgly5 carrying pTrc99A-aceAK-ycdW and pCDF-gltA [5]
 Mgly624 Mgly6 carrying pTrc99A-aceA-ycdW and pCDF-gltA This study
 Mgly6241 Mgly6 carrying pTrc99A-aceA-ycdW, pCDF-gltA and pRSF-argT This study
 Mgly6242 Mgly6 carrying pTrc99A-aceA-ycdW, pCDF-gltA and pCOLA-argT This study
Plasmids
 pKD4 oriR6Kγ, KanR, rgnB (Ter) [52]
 pKD46 araBp-gam-bet-exo, bla (AmpR), repA101 (ts), ori101 [52]
 pCP20 AmpR, CmR, FLP recombinance oriR6Kγ, KanR, rgnB (Ter) [53]
 pTrc99A-aceA-ycdW AmpR, pTrc99A harboring aceA and ycdW from E. coli MG1655 This study
 pTrc99A-aceAK-ycdW AmpR, pTrc99A harboring aceA-aceK and ycdW from E. coli MG1655 [5]
 pCDF-gltA StrepR, pCDFDuet-1 harboring gltA from E. coli MG1655 [5]
 pRSF-argT KanR, pRSFDuet-1 harboring argT from E. coli MG1655 This study
 pCOLA-argT KanR, pCOLADuet-1 harboring argT from E. coli MG1655 This study