Table 1 Strains and plasmids used in this study

 MG1655(DE3)

fhuA2

[51]

 Mgly5

MG1655(DE3)ΔldhAΔglcBΔaceBΔaldAΔglcDEF

[5]

 Mgly6

MG1655(DE3)ΔldhAΔglcBΔaceBΔaldAΔglcDEFΔicd

This study

 Mgly534

Mgly5 carrying pTrc99A-aceAK-ycdW and pCDF-gltA

[5]

 Mgly624

Mgly6 carrying pTrc99A-aceA-ycdW and pCDF-gltA

This study

 Mgly6241

Mgly6 carrying pTrc99A-aceA-ycdW, pCDF-gltA and pRSF-argT

This study

 Mgly6242

Mgly6 carrying pTrc99A-aceA-ycdW, pCDF-gltA and pCOLA-argT

This study

 pKD4

oriR6Kγ, Kan^R, rgnB (Ter)

[52]

 pKD46

araBp-gam-bet-exo, bla (Amp^R), repA101 (ts), ori101

[52]

 pCP20

Amp^R, Cm^R, FLP recombinance oriR6Kγ, Kan^R, rgnB (Ter)

[53]

 pTrc99A-aceA-ycdW

Amp^R, pTrc99A harboring aceA and ycdW from E. coli MG1655

This study

 pTrc99A-aceAK-ycdW

Amp^R, pTrc99A harboring aceA-aceK and ycdW from E. coli MG1655

[5]

 pCDF-gltA

Strep^R, pCDFDuet-1 harboring gltA from E. coli MG1655

[5]

 pRSF-argT

Kan^R, pRSFDuet-1 harboring argT from E. coli MG1655

This study

 pCOLA-argT

Kan^R, pCOLADuet-1 harboring argT from E. coli MG1655

This study