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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Transplantation of CRISPRa system engineered IL10-overexpressing bone marrow-derived mesenchymal stem cells for the treatment of myocardial infarction in diabetic mice

Fig. 5

Transplantation of BM-MSCs-pCas9/gIL10 alleviates cardiac injury, decreases apoptosis of cardiac cells and increases angiogenesis in mice model of diabetic myocardial infarction. a Representative Masson’s trichrome–stained myocardial sections in the control MI heart or hearts treated with BM-MSCs pCas9/gCtrl, pCas9/gIL10 or BM-MSCs pCas9/gIL10. Blue, scar tissue; red, viable myocardium. b Quantitation of infarct size in the four indicated experimental groups. Data represent means ± SD. **p < 0.005, n = 8. c Representative fluorescent micrographs showing the presence of TUNEL+ (terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling–positive) apoptotic cells (green) in the border zone of LV infarct. Nuclei were stained by DAPI (blue). d Quantification of percentage of apoptotic cells in the four indicated experimental groups. Data represent means ± SD. **p < 0.005, n = 8. e Angiogenesis was inspected using von Willebrand factor (vWF) staining in heart sections from different groups 4 weeks after MI and transplantation. Tissue sections were stained primary rabbit anti-vWF antibody and secondary goat anti-Rabbit IgG antibody labeled with Alexa Fluor 647 (red). f Summary of total vessel density in different groups. Data represent means ± SD. *p < 0.05, **p < 0.005, n = 8

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