Fig. 4

(a) Effects of temperature and concentration of the inducer IPTG on the lipoylation of H protein expressed in vivo; (b) Effect of lipoic acid concentration added into the culture on the lipoylation of H protein (lane 1–5, H protein was expressed in pET28a; lane 6, H protein and LplA was co-expressed in pETduet; the lipoic acid concentration added in this case was 20 μM)